Publications
Special Edition - Call for Submissions
Submissions are being accepted for the Special Issue of Biomolecules, an open-access journal by MDPI, now through July 31 2026. Our chief scientific officer, Eric B. Kmiec, and associate director of discovery research, Kelly Banas, are guest editors for this special issue, which promises to explore CRISPR gene editing at every stage of action, be it mechanistic or translational. Topics of interest include molecular insights of CRISPR/Cas systems in cancer-based models; novel approaches for altering the tumor microenvironment or disrupting oncogenic pathways; strategies for improving safety, efficiency, or precision of CRISPR/Cas systems for cancer therapy; and diagnostic applications of CRISPR technologies as tools for detection or molecular profiling.
2026
Target choice and exon skipping regulate CRISPR-directed gene editing of NRF2 in head/neck and esophageal cancer cells
Authors: Rivera-Torres, N; Skelly, LE; Rogowskyj, JA; Aguilar, G; Banas, KH; Bialk, PA; Kmiec, EB
Journal: Molecular Therapy Oncology
2025
Functional characterization of tumor-specific CRISPR-directed gene editing as a combinatorial therapy for the treatment of solid tumors
Authors: Banas, KH; Bialk, PA; Rivera-Torres, N; Owens, K; Reiner, TN; Pisarcik, KM; Haas, N; Gielda, E; Khan, K; Narra, KP; Kmiec, EB
Journal: Molecular Therapy Oncology
CRISPR-directed exon skipping; friend or foe (opportunity or warning sign)?
Authors: Banas, KH; Kmiec, EB
Journal: Molecular Therapy Nucleic Acids
2024
Mutation-Specific CRISPR Targeting with SaCas9 and AsCas12a Restores Therapeutic Sensitivity in Treatment-Resistant Melanoma
Authors: Sansbury, BM., Masciarelli SB., Kaouser S, Tharp OM, Banas KH & Kmiec EB
Journal: The CRISPR Journal
Looking Forward
Authors: Kmiec EB
Journal: Gene
CRISPR-directed gene-editing induces genetic rearrangement within the human globin gene locus
Authors: Tharp O, Sansbury BM, & Kmiec E
Journal: Gene
2023
Learning Lab: A Hands-On Way for Future Scientists to Engage with CRISPR
Authors: Hewes A, LaTorre S, Sisson M, & Hake D
Journal: Delaware Journal of Public Health
CRISPR-Directed Gene Editing as a Method to Reduce Chemoresistance in Lung Cancer Cells
Authors: Rivera-Torres N, Bialk P & Kmiec EB
Journal: Cancer Systems and Integrative Biology
2022
An Updated Stepped Elongation Time (STeP-Up) Sanger Sequencing Protocol to Economize Workflows and Increase Useability
Authors: Masciarelli S, Tharp O, Sansbury B
Journal: Protocol Exchange
Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas
Authors: Sansbury BM, Hewes AM, Tharp OM, Masciarelli SB, Kaouser S, Kmiec EB
Journal: Nature, Scientific Reports, 2022
Exon skipping induced by CRISPR-directed gene editing regulates the response to chemotherapy in non-small cell lung carcinoma cells
Authors: Banas K, Modarai S, Rivera-Torres N, Yoo B-C, Bialk P, Barrett C, Batish M, Kmiec EB
Journal: Gene Therapy, 2022
2021
Development of Cell Lines for use in COVID-19 Drug Development and Research
Authors: London McGill
Journal: Delaware Journal of Public Health
Recent Scientific Breakthroughs Applying CRISPR Gene Editing in Neurological Disorders
Authors: Mitra Assadi-Khansari & London Pamela McGill
Journal: Delaware Journal of Public Health
CRISPR In A Box™ and the Journey Toward Inspiring New Scientists, Kristen Pisarcik, Delaware Journal of Public Health
Authors: Kristen Pisarcik
Journal: Delaware Journal of Public Health
Precise and error-prone CRISPR-directed gene editing activity in human CD34+ cells varies widely among patient samples
Authors: Modarai S, Kanda S, Bloh K, Opdenaker L, Kmiec EB
Journal: Gene Therapy, 2021
A complete methodology for the instruction of CRISPR-based gene editing using a simplified cell-free extract system with genetic readout in bacteria
Authors: Pisarcik K, Sansbury BM, Kmiec EB
Journal: CRISPR-Cas Methods. Springer Protocols Handbooks, 2021
On the Origins of Homology Directed Repair in Mammalian Cells
Authors: Sansbury B, Kmiec EB
Journal: International Journal of Molecular Sciences, 2021
Deconvolution of complex DNA repair DECODR: Establishing a novel deconvolution algorithm for comprehensive analysis of CRISPR-edited Sanger sequencing data
Authors: Bloh K, Kanchana R, Bialk P, Zhang Z, Yoo, B-C, Kmiec EB
Journal: CRISPR Journal, 2021
A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity
Authors: Bloh K, Rivera-Torres N
Journal: International Journal of Molecular Sciences, 2021
A toolmaker’s perspective on CRISPR-directed gene editing as a therapeutic strategy for leukemia and beyond
Authors: Kmiec EB, Bloh K
Journal: Expert Reviews in Hematology, 2021
2020
The diversity of genetic outcomes from CRISPR/Cas gene editing is regulated by the length of the symmetrical donor DNA template
Authors: Hewes AM, Sansbury BM, Kmiec EB
Journal: Genes (Tenth Anniversary Issue), 2020
Modeling pediatric AML FLT3 mutations using CRISPR/Cas12a- mediated gene editing
Authors: Rivera-Torres N, Banas K, Kmiec EB
Journal: Leukemia & Lymphoma, 2020
gRNA sequence heterology tolerance catalyzed by CRISPR/Cas in an in vitro homology directed repair reaction
Authors: Hewes AM, Sansbury BM, Barth S, Tarcic G, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2020
Potential Inequities in New Medical Technologies
Authors: Kmiec EB, Marron J
Journal: Scientific American, 2020
Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells
Authors: Banas K, Rivera-Torres N, Bialk P, Yoo BC, Kmiec EB
Journal: Molecular Cancer Research, 2020
2019
Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
Authors: Sansbury BM, Hewes AM, Kmiec EB
Journal: Nature, Communications Biology, 2019
CRISPR-Directed Gene Editing Catalyzes Precise Gene Segment Replacement In Vitro Enabling a Novel Method for Multiplex Site-Directed Mutagenesis
Authors: Sansbury B, Wagner AM, Tarcic G, Barth S, Nitzan E, Goldfus R, Vidne M, Kmiec EB
Journal: The CRISPR Journal
CRISPR-Directed Gene Editing in a Community Cancer Center
Authors: Kmiec EB
Journal: Oncology Issues, 2019
2018
Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells in Vitro and in Xenograft Mouse Model
Authors: Bialk P, Wang Y, Banas K, Kmiec EB
Journal: Molecular Therapy Oncolytics, 2018
Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex
Authors: Modarai SR, Man D, Bialk P, Rivera-Torres N, Bloh K, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2018
CRISPR-directed gene editing in vitro catalyzed by Cpf1 (Cas12a) nuclease and a mammalian cell-free extract
Authors: Sansbury BM, Wagner AM, Nitzan E, Tarcic G, Kmiec EB
Journal: CRISPR Journal, 2018
2017
A Standard Methodology to Examine On-site Mutagenecity as a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and ssODN in Human Cells
Authors: Rivera-Torres N, Kmiec EB
Journal: Journal of Visualized Experiments (JoVE), 2017
Allelic analysis of on-target CRISPR/Cas9 heterogeneity by the focused disruption and reassignment of the GATA1 initiation codon in ML-DS
Authors: Bloh K, Bialk P, Gopalakrishnapillai A, Kolb EA, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2017
To Cleave or Not to Cleave: Therapeutic Editing with and without Programmable Nucleases
Authors: Woolf TM, Channabasavaiah GB, Boyce F, Kmiec EB
Journal: Nature Reviews Drug Discovery, 2017
Insertional mutagenesis by CRISPR/Cas9 Ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides
Authors: Rivera-Torres N, Banas K, Bialk PB, Bloh K, Kmiec EB
Journal: PLOS-ONE, 2017
2016
Personalized models reveal mechanistic and therapeutic insights into CEP290-associated Leber congenital amaurosis
Authors: McDougald DS, Kmiec EB, Mills JA
Journal: Stem Cell Investigation, 2016
Gene Editing for Cancer is Coming of Age
Authors: Kmiec EB
Journal: Oncology Times, 2016
Analysis of point mutation repair and allelic heterogeneity generated by CRISP/Cas9 and single-stranded DNA oligonucleotides
Authors: Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Dula M, Kmiec EB
Journal: Nature, Scientific Reports, 2016
2015
Genetic spellchecking: gene editing using single-stranded DNA oligonucleotides
Authors: Rivera-Torres N, Kmiec EB
Journal: Plant Biotech Journal, 2015
Regulation of gene editing activity directed by single-stranded oligonucleotides and CRISPR/Cas9 systems
Authors: Bialk P, Rivera-Torres N, Strouse B, Kmiec EB
Journal: PLOS-ONE, 2015
Is the age of genetic surgery finally upon us?
Authors: Kmiec EB
Journal: Surgical Oncology, 2015
2014
The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-Stranded Oligonucleotides
Authors: Rivera-Torres N, Strouse B, Bialk P, Niamat R and Kmiec EB
Journal: PLOS-ONE, 2014
Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds
Authors: Sims-Mourtada J, Niamat R, Samuel S, Eskridge C and Kmiec EB
Journal: International Journal of Nanomedicine, 2014
Combinatorial gene editing in mammalian cells using ssODNs and TALENs
Authors: Strouse B, Bialk P, Niamat R, Rivera-Torres N and Kmiec EB
Journal: Nature, Scientific Reports, 2014
2013
Electrospun fiber membranes enable proliferation of genetically modified cells
Authors: Borjigin M, Eskridge C, Niamat, R, Strouse, B, Bialk P and Kmiec EB
Journal: International Journal of Nanomedicine, 2013
2012
Proliferation of Genetically Modified Human Cells on Electrospun Nanofiber Scaffolds
Authors: Borjigin M, Strouse B, Niamat R, Bialk P, Eskridge C, Xie J and Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2012
Oligonucleotide Delivery by Nucleofection Does Not Rescue the Reduced Proliferation Phenotype of Gene-Edited Cells
Authors: Livingston P, Strouse B, Perry H, Borjigin M, Bialk P and Kmiec EB
Journal: Nucleic Acid Therapeutics, 2012
