Publications
2024
Mutation-Specific CRISPR Targeting with SaCas9 and AsCas12a Restores Therapeutic Sensitivity in Treatment-Resistant Melanoma
Authors: Sansbury, BM., Masciarelli SB., Kaouser S, Tharp OM, Banas KH & Kmiec EB
Journal: The CRISPR Journal
Looking Forward
Authors: Kmiec EB
Journal: Gene
CRISPR-directed gene-editing induces genetic rearrangement within the human globin gene locus
Authors: Tharp O, Sansbury BM, & Kmiec E
Journal: Gene
2023
Learning Lab: A Hands-On Way for Future Scientists to Engage with CRISPR
Authors: Hewes A, LaTorre S, Sisson M, & Hake D
Journal: Delaware Journal of Public Health
CRISPR-Directed Gene Editing as a Method to Reduce Chemoresistance in Lung Cancer Cells
Authors: Rivera-Torres N, Bialk P & Kmiec EB
Journal: Cancer Systems and Integrative Biology
2022
An Updated Stepped Elongation Time (STeP-Up) Sanger Sequencing Protocol to Economize Workflows and Increase Useability
Authors: Masciarelli S, Tharp O, Sansbury B
Journal: Protocol Exchange
Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas
Authors: Sansbury BM, Hewes AM, Tharp OM, Masciarelli SB, Kaouser S, Kmiec EB
Journal: Nature, Scientific Reports, 2022
Exon skipping induced by CRISPR-directed gene editing regulates the response to chemotherapy in non-small cell lung carcinoma cells
Authors: Banas K, Modarai S, Rivera-Torres N, Yoo B-C, Bialk P, Barrett C, Batish M, Kmiec EB
Journal: Gene Therapy, 2022
2021
Development of Cell Lines for use in COVID-19 Drug Development and Research
Authors: London McGill
Journal: Delaware Journal of Public Health
Recent Scientific Breakthroughs Applying CRISPR Gene Editing in Neurological Disorders
Authors: Mitra Assadi-Khansari & London Pamela McGill
Journal: Delaware Journal of Public Health
CRISPR In A Box™ and the Journey Toward Inspiring New Scientists, Kristen Pisarcik, Delaware Journal of Public Health
Authors: Kristen Pisarcik
Journal: Delaware Journal of Public Health
Precise and error-prone CRISPR-directed gene editing activity in human CD34+ cells varies widely among patient samples
Authors: Modarai S, Kanda S, Bloh K, Opdenaker L, Kmiec EB
Journal: Gene Therapy, 2021
A complete methodology for the instruction of CRISPR-based gene editing using a simplified cell-free extract system with genetic readout in bacteria
Authors: Pisarcik K, Sansbury BM, Kmiec EB
Journal: CRISPR-Cas Methods. Springer Protocols Handbooks, 2021
On the Origins of Homology Directed Repair in Mammalian Cells
Authors: Sansbury B, Kmiec EB
Journal: International Journal of Molecular Sciences, 2021
Deconvolution of complex DNA repair DECODR: Establishing a novel deconvolution algorithm for comprehensive analysis of CRISPR-edited Sanger sequencing data
Authors: Bloh K, Kanchana R, Bialk P, Zhang Z, Yoo, B-C, Kmiec EB
Journal: CRISPR Journal, 2021
A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity
Authors: Bloh K, Rivera-Torres N
Journal: International Journal of Molecular Sciences, 2021
A toolmaker’s perspective on CRISPR-directed gene editing as a therapeutic strategy for leukemia and beyond
Authors: Kmiec EB, Bloh K
Journal: Expert Reviews in Hematology, 2021
2020
The diversity of genetic outcomes from CRISPR/Cas gene editing is regulated by the length of the symmetrical donor DNA template
Authors: Hewes AM, Sansbury BM, Kmiec EB
Journal: Genes (Tenth Anniversary Issue), 2020
Modeling pediatric AML FLT3 mutations using CRISPR/Cas12a- mediated gene editing
Authors: Rivera-Torres N, Banas K, Kmiec EB
Journal: Leukemia & Lymphoma, 2020
gRNA sequence heterology tolerance catalyzed by CRISPR/Cas in an in vitro homology directed repair reaction
Authors: Hewes AM, Sansbury BM, Barth S, Tarcic G, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2020
Potential Inequities in New Medical Technologies
Authors: Kmiec EB, Marron J
Journal: Scientific American, 2020
Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells
Authors: Banas K, Rivera-Torres N, Bialk P, Yoo BC, Kmiec EB
Journal: Molecular Cancer Research, 2020
2019
Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
Authors: Sansbury BM, Hewes AM, Kmiec EB
Journal: Nature, Communications Biology, 2019
CRISPR-Directed Gene Editing Catalyzes Precise Gene Segment Replacement In Vitro Enabling a Novel Method for Multiplex Site-Directed Mutagenesis
Authors: Sansbury B, Wagner AM, Tarcic G, Barth S, Nitzan E, Goldfus R, Vidne M, Kmiec EB
Journal: The CRISPR Journal
CRISPR-Directed Gene Editing in a Community Cancer Center
Authors: Kmiec EB
Journal: Oncology Issues, 2019
2018
Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells in Vitro and in Xenograft Mouse Model
Authors: Bialk P, Wang Y, Banas K, Kmiec EB
Journal: Molecular Therapy Oncolytics, 2018
Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex
Authors: Modarai SR, Man D, Bialk P, Rivera-Torres N, Bloh K, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2018
CRISPR-directed gene editing in vitro catalyzed by Cpf1 (Cas12a) nuclease and a mammalian cell-free extract
Authors: Sansbury BM, Wagner AM, Nitzan E, Tarcic G, Kmiec EB
Journal: CRISPR Journal, 2018
2017
A Standard Methodology to Examine On-site Mutagenecity as a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and ssODN in Human Cells
Authors: Rivera-Torres N, Kmiec EB
Journal: Journal of Visualized Experiments (JoVE), 2017
Allelic analysis of on-target CRISPR/Cas9 heterogeneity by the focused disruption and reassignment of the GATA1 initiation codon in ML-DS
Authors: Bloh K, Bialk P, Gopalakrishnapillai A, Kolb EA, Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2017
To Cleave or Not to Cleave: Therapeutic Editing with and without Programmable Nucleases
Authors: Woolf TM, Channabasavaiah GB, Boyce F, Kmiec EB
Journal: Nature Reviews Drug Discovery, 2017
Insertional mutagenesis by CRISPR/Cas9 Ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides
Authors: Rivera-Torres N, Banas K, Bialk PB, Bloh K, Kmiec EB
Journal: PLOS-ONE, 2017
2016
Personalized models reveal mechanistic and therapeutic insights into CEP290-associated Leber congenital amaurosis
Authors: McDougald DS, Kmiec EB, Mills JA
Journal: Stem Cell Investigation, 2016
Gene Editing for Cancer is Coming of Age
Authors: Kmiec EB
Journal: Oncology Times, 2016
Analysis of point mutation repair and allelic heterogeneity generated by CRISP/Cas9 and single-stranded DNA oligonucleotides
Authors: Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Dula M, Kmiec EB
Journal: Nature, Scientific Reports, 2016
2015
Genetic spellchecking: gene editing using single-stranded DNA oligonucleotides
Authors: Rivera-Torres N, Kmiec EB
Journal: Plant Biotech Journal, 2015
Regulation of gene editing activity directed by single-stranded oligonucleotides and CRISPR/Cas9 systems
Authors: Bialk P, Rivera-Torres N, Strouse B, Kmiec EB
Journal: PLOS-ONE, 2015
Is the age of genetic surgery finally upon us?
Authors: Kmiec EB
Journal: Surgical Oncology, 2015
2014
The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-Stranded Oligonucleotides
Authors: Rivera-Torres N, Strouse B, Bialk P, Niamat R and Kmiec EB
Journal: PLOS-ONE, 2014
Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds
Authors: Sims-Mourtada J, Niamat R, Samuel S, Eskridge C and Kmiec EB
Journal: International Journal of Nanomedicine, 2014
Combinatorial gene editing in mammalian cells using ssODNs and TALENs
Authors: Strouse B, Bialk P, Niamat R, Rivera-Torres N and Kmiec EB
Journal: Nature, Scientific Reports, 2014
2013
Electrospun fiber membranes enable proliferation of genetically modified cells
Authors: Borjigin M, Eskridge C, Niamat, R, Strouse, B, Bialk P and Kmiec EB
Journal: International Journal of Nanomedicine, 2013
2012
Proliferation of Genetically Modified Human Cells on Electrospun Nanofiber Scaffolds
Authors: Borjigin M, Strouse B, Niamat R, Bialk P, Eskridge C, Xie J and Kmiec EB
Journal: Molecular Therapy – Nucleic Acids, 2012
Oligonucleotide Delivery by Nucleofection Does Not Rescue the Reduced Proliferation Phenotype of Gene-Edited Cells
Authors: Livingston P, Strouse B, Perry H, Borjigin M, Bialk P and Kmiec EB
Journal: Nucleic Acid Therapeutics, 2012
