Drs. Eric Kmiec and Brett Sansbury will be guest editors of the International Journal of Molecular Sciences Special Issue “Cellular and Molecular Mechanisms of CRISPR-Directed Gene Repair” (Read more)
Read Drs. Eric Kmiec and Jonathan Marron’s latest Op-Ed in Scentific American, “Risks in Breakthrough Technologies”
Kmiec EB, Bloh K (2021) A toolmaker’s perspective on CRISPR-directed gene editing as a therapeutic strategy for leukemia and beyond. Expert Reviews in Hematology (DOI: 10.1080/17474086.2021.1935853)
Bloh K, Rivera-Torres N (2021) A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity. International Journal of Molecular Sciences (DOI: 10.3390/ijms22083834)
Sansbury BM, Kmiec EB (2021) On the Origins of Homology Directed Repair in Mammalian Cells. International Journal of Molecular Sciences (DOI: 10.3390/ijms22073348)
Bloh K, Kanchana R, Bialk P, Banas K, Zhang Z, Yoo BC, Kmiec EB (2021) Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger Sequencing Data. CRISPR Journal (DOI: 10.1089/crispr.2020.0022)
Hewes AM, Sansbury BM, Kmiec EB (2020) The Diversity of Genetic Outcomes from CRISPR/Cas Gene Editing is Regulated by the Length of the Symmetrical Donor DNA Template. Genes (DOI: 10.3390/genes11101160)
Modarai SR, Kanda S, Bloh K, Opdenaker LM, Kmiec EB (2020) Precise and error-prone CRISPR-directed gene editing activity in human CD34+ cells varies widely among patient samples. Gene Therapy (DOI: 10.1038/s41434-020-00192-z)
Rivera-Torres N, Banas K, Kmiec EB (2020) Modeling pediatric AML FLT3 mutations using CRISPR/Cas12a- mediated gene editing. Leukemia & Lymphoma (DOI: 10.1080/10428194.2020.1805740)
Hewes AM, Sansbury BM, Barth S, Tarcic G, Kmiec EB (2020) gRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction. Molecular Therapy-Nucleic Acids (DOI: 10.1016/j.omtn.2020.03.012)
Banas K, Rivera-Torres N, Bialk P, Yoo BC, Kmiec EB (2020) Kinetics of Nuclear Uptake an Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells. Molecular Cancer Research (DOI: 10.1158/1541-7786.MCR-19-1208).
Hewes AM, Sansbury BM, Barth S, Tarcic G, Kmiec EB (2020) gRNA sequence heterology tolerance catalyzed by CRISPR/Cas in an in vitro homology directed repair reaction. Molecular Therapy-Nucleic Acids, 20 568-579 (doi.org/10.1016/j.omtn.2020.03.012)
Sansbury BM, Hewes AM, Kmiec EB (2019) Understanding the diversity of genetic outcomes from homology directed repair activity generated by CRISPR-Cas gene editing. Nature Communications, Biology.2, 458. (doi.org/10.1038/s42003-019-0705-y)
Kmiec EB (2019) CRISPR-Directed Gene Editing in a Community Cancer Center, Oncology Issues, 34:1, 31-37, (DOI: 10.1080/10463356.2018.1553424).
Bialk P, Wang Y, Banas K, Kmiec EB (2018) Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells In Vitro and in a Xenograft Mouse Model, Molecular Therapy – Oncolytics. (DOI: 10.1016/j.omto.2018.10.002).
Modarai SR, Man D, Bialk P, Rivera-Torres N, Bloh K, Kmiec EB (2018) Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex, Molecular Therapy – Nucleic Acids. (DOI: 10.1016/j.omtn.2018.01.013).
Sansbury BM, Wagner AM, Nitzan E, Tarcic G, Kmiec EB (2018) CRISPR-directed gene editing in vitro catalyzed by Cpf1 (Cas12a) nuclease and a mammalian cell-free extract. CRISPR Journal (doi.org/10.1089/crispr.2018.0006).
Rivera-Torres N, Kmiec EB (2017) A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells, Journal of Visualized Experiments. (DOI: 10.3791/56195).
Bloh K, Bialk P, Gopalakrishnapillai A, Kolb EA, Kmiec EB (2017) Allelic analysis of on-target CRISPR/Cas9 heterogeneity by the focused disruption and reassignment of the GATA1 initiation codon in ML-DS, Molecular Therapy – Nucleic Acids. (DOI: 10.1016/j.omtn.2017.04.009).
Woolf TM, Channabasavaiah GB, Boyce F, Kmiec EB (2017) To Cleave or Not to Cleave: Therapeutic Editing with and without Programmable Nucleases. Nature, Drug Development (DOI: 10.1038/nrd.2017.42).
Rivera-Torres N, Banas K, Bialk PB, Bloh K, Kmiec EB (2017) Insertional mutagenesis by CRISPR/Cas9 Ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides. PLOS ONE (DOI: 10.1371/journal.pone.0169350).
McDougald DS, Kmiec EB, Mills JA (2016) Personalized models reveal mechanistic and therapeutic insights into CEP290-associated Leber congenital amaurosis. Stem Cell (DOI: 10.21037/sci.2016.10.03).
Kmiec EB (2016) Gene Editing for Cancer is Coming of Age. Oncology Times (DOI: 10.1097/01.COT.0000502644.42641.e4).
Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Dula M, Kmiec EB (2016) Analysis of point mutation repair and allelic heterogeneity generated by CRISP/Cas9 and single-stranded DNA oligonucleotides. Nature: Sci. Rep. (DOI: 10. 1038/srep32681).
Rivera-Torres N, Kmiec EB (2015) Genetic spellchecking: gene editing using single-stranded DNA oligonucleotides. Plant Biotech Journal (DOI: 10.1111-1-8).
Bialk, P., Rivera-Torres, N., Strouse, B., & Kmiec, E. B. (2015). Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. PLoS ONE, 10(6), e0129308.
Kmiec EB (2015) Is the age of genetic surgery finally upon us? Surgical Oncology 24:95-99.
Strouse B, Bialk P, Niamat R, Rivera-Torres N and Kmiec EB (2014) Combinatorial gene editing in mammalian cells using ssODNs and TALENs. Nature; Sci. Rep. 4:379, 1-9.
Rivera-Torres N, Strouse B, Bialk P, Niamat R and Kmiec EB (2014) Strand bias and proximal cleavage by TALENs influence the frequency of gene editing directed by ssODNs. PLOS-ONE 9:5, e96483.
Borjigin M, Strouse B, Niamat R, Bialk P, Eskridge C, Xie J and Kmiec EB (2012) Proliferation of Genetically Modified Human Cells on Electrospun Nanofiber Scaffolds. Mol Ther Nucleic Acids Dec 4; 1:e59.
Bonner M, Livingston P, Applegate M and Kmiec EB (2012). DNA Damage Response and Replication Fork Stress During Oligonucleotide Directed Gene Editing Molecular Therapy 1, e18: 3 April, 2012 (1-14).
Engstrom J and Kmiec EB (2008) DNA Replication, Cell Cycle Progression and the Targeted Gene Repair Reaction. Cell Cycle Feb 28;7(10).
Ferrara L and Kmiec EB (2006) Targeted gene repair activates Chk1 and Chk2 and stalls replication in corrected cells. DNA Repair (Amst). 2006 Apr 8;5(4):422-31. Epub 2006 Jan 18.
Brachman E and Kmiec EB (2005) Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks. DNA Repair 4, 445-457.
Ferrara F and Kmiec EB (2004) Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination. Nucleic Acids Research. 32(17), 5239-5248.
Brachman E and Kmiec EB (2003) Targeted nucleotide repair of cyc1 mutations in Saccharomyces cerevisiae directed by modified single-stranded DNA oligonucleotides. Genetics 163, 2, 527-538.
Liu L, Parekh-Olmedo H and Kmiec EB (2003) Development and regulation of gene repair. Nature Reviews Genetics 4, 679-689.