Genome Editing
Research Planning Expertise
Let our experts help your researchers plan and execute their translational genome editing experiments.

Genome Editing
Operating within ChristianaCare, a community health care provider in Wilmington, Delaware, the pioneering Gene Editing Institute is a patient-first, innovative research organization that has evolved into an acknowledged global leader in the field of gene editing for human impact.
The Institute has produced several firsts, including the development of the first CRISPR tool to repair DNA outside the human cell to speed therapies to patients.
Contact the Gene Editing Institute today to discuss how we can put our expertise to work to advance your research.
Specific Service Offerings
Knock Out Gene Editing Services
- – Single gene knockout
- – Multi-gene knockout
- – Large deletion
- – Knockout pools
Knock In Gene Editing Services
- – Point mutation/insertional mutation (i.e. epitope tagging)
- – Large insertional mutation (i.e. fluorescent protein fusion, transcriptional termination, etc.)
- – Multi-gene knock in
For all genome editing services, the Gene Editing Institute offers reliable gene editing reagents, cell lines, and supporting data.
Deliverables
- – Two independent clonal cell lines (2 vials of 1M cells)
- – Parental cell lines (1M cells)
- – Sequence analysis
- – Raw sequence files
- – Certificate of analysis
Timeline

The Gene Editing Institute provides highly customized CRISPR Genome Editing services to meet your basic research, translational, and clinical needs.
Custom Project Workflow
1.
Project Design and Assessment
- - Custom cell line sterility verification
- - gRNA(s) and HDR template design and synthesis
2.
Reagent Synthesis
- - PCR design
- - Primer synthesis
- - Reaction optimization
3.
Transfection & Bulk Analysis
- - Cell transfections
- - Bulk population PCR and Sanger sequencing
- - Bulk population isolation (if requested)
4.
Clonal Sorting/Expansion
- - Single-cell sorting via FACS
- - Clonal expansion and preparation
5.
Clone Verification
- - Clonal genomic analysis by Sanger sequencing and DECODR analysis (for on-target verification)*
- - Cell banking
6.
Delivery
- – Delivery of identified engineered clones and supporting data
*Upon request, the Institute can provide additional services to further evaluate gene editing efficacy at the DNA and protein levels via Western Blot, ELISA or relevant functional assay.
Case Studies




Need more information?
To find out more, email us at geneeditinginstitute@christianacare.org or call 302-623-5306.