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Genome Editing

Research Planning Expertise

Let our experts help your researchers plan and execute their translational genome editing experiments.

Genome Editing

Operating within ChristianaCare, a community health care provider in Wilmington, Delaware, the pioneering Gene Editing Institute is a patient-first, innovative research organization that has evolved into an acknowledged global leader in the field of gene editing for human impact.

The Institute has produced several firsts, including the development of the first CRISPR tool to repair DNA outside the human cell to speed therapies to patients.

Contact the Gene Editing Institute today to discuss how we can put our expertise to work to advance your research.

Specific Service Offerings

Knock Out Gene Editing Services
  • Single gene knockout
  • Multi-gene knockout
  • Large deletion
  • Knockout pools
Knock In Gene Editing Services
  • Point mutation/insertional mutation (i.e. epitope tagging)
  •  Large insertional mutation (i.e. fluorescent protein fusion, transcriptional termination, etc.)
  • Multi-gene knock in

For all genome editing services, the Gene Editing Institute offers reliable gene editing reagents, cell lines, and supporting data.

Deliverables
  • Two independent clonal cell lines (2 vials of 1M cells)
  • Parental cell lines (1M cells)
  • Sequence analysis
  • Raw sequence files
  • Certificate of analysis

Timeline

CRISPR technology timeline

The Gene Editing Institute provides highly customized CRISPR Genome Editing services to meet your basic research, translational, and clinical needs.

Custom Project Workflow

1.
Project Design and Assessment

- Custom cell line sterility verification
- gRNA(s) and HDR template design and synthesis

2.
Reagent Synthesis

- PCR design
- Primer synthesis
- Reaction optimization

3.
Transfection & Bulk Analysis

- Cell transfections
- Bulk population PCR and Sanger sequencing
- Bulk population isolation (if requested)

4.
Clonal Sorting/Expansion

- Single-cell sorting via FACS
- Clonal expansion and preparation

5.
Clone Verification

- Clonal genomic analysis by Sanger sequencing and DECODR analysis (for on-target verification)*
- Cell banking

6.
Delivery

– Delivery of identified engineered clones and supporting data

*Upon request, the Institute can provide additional services to further evaluate gene editing efficacy at the DNA and protein levels via Western Blot, ELISA or relevant functional assay.

Case Studies

Strategy: Target all known splicing variants of ITGβ6 by choosing appropriate gRNA to ensure effective knockout (A). TIDE analysis of the transfected cells (bulk population) shows high knockout efficiency (B). TIDE analysis of a clonally expanded cell shows that all three copies of ITGβ6 are knocked-out via frame-shifting indels (C).

Homozygous knockout diagram

Strategy: Target all known splicing variants of KEAP1 (red text) to ensure effective knockout using two gRNAs simultaneously (A). TIDE analyses of a clone shows frame-shifting indels at both CRISPR targeted loci, generating a knockout of all variants (B, C).

TEST

Strategy: Induce two amino acid changes using CRISPR and HDR template DNA (A). Clonal analysis shows successful homozygous mutations were created at the intended sites (B).

pointmutation

Strategy: Knock in 24bp epitope tag sequence at C-terminus using CRISPR and HDR template DNA (A). Clonal analysis shows homozygous in-frame insertion of the 24bp epitope tag sequence at the C-terminus (B).

epitope

Need more information?

To find out more, email us at geneeditinginstitute@christianacare.org or call
302-623-5306.