Publications

2022

An Updated Stepped Elongation Time (STeP-Up) Sanger Sequencing Protocol to Economize Workflows and Increase Useability

Authors: Sophia B. Masciarelli, Olivia M. Tharp, Brett M. Sansbury

Journal: ChristianaCare, Gene Editing Institute

Exon skipping induced by CRISPR-directed gene editing regulates the response to chemotherapy in non-small cell lung carcinoma cells

Authors: Banas K, Modarai S, Rivera-Torres N, Yoo B-C, Bialk P, Barrett C, Batish M, Kmiec EB

Journal: Gene Therapy, 2022

2021

Development of Cell Lines for use in COVID-19 Drug Development and Research

Authors: London McGill

Journal: Delaware Journal of Public Health

Recent Scientific Breakthroughs Applying CRISPR Gene Editing in Neurological Disorders

Authors: Mitra Assadi-Khansari & London Pamela McGill

Journal: Delaware Journal of Public Health

CRISPR In A Box™ and the Journey Toward Inspiring New Scientists, Kristen Pisarcik, Delaware Journal of Public Health

Authors: Kristen Pisarcik

Journal: Delaware Journal of Public Health

Precise and error-prone CRISPR-directed gene editing activity in human CD34+ cells varies widely among patient samples

Authors: Modarai S, Kanda S, Bloh K, Opdenaker L, Kmiec EB

Journal: Gene Therapy, 2021

A complete methodology for the instruction of CRISPR-based gene editing using a simplified cell-free extract system with genetic readout in bacteria

Authors: Pisarcik K, Sansbury BM, Kmiec EB

Journal: CRISPR-Cas Methods. Springer Protocols Handbooks, 2021

On the Origins of Homology Directed Repair in Mammalian Cells

Authors: Sansbury S, Kmiec EB

Journal: International Journal of Molecular Sciences, 2021

Deconvolution of complex DNA repair DECODR: Establishing a novel deconvolution algorithm for comprehensive analysis of CRISPR-edited Sanger sequencing data

Authors: Bloh K, Kanchana R, Bialk P, Zhang Z, Yoo, B-C, Kmiec EB

Journal: CRISPR Journal, 2021

A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity

Authors: Sansbury S, Kmiec EB

Journal: International Journal of Molecular Sciences, 2021

A toolmaker’s perspective on CRISPR-directed gene editing as a therapeutic strategy for leukemia and beyond

Authors: Kmiec EB, Bloh K

Journal: Expert Reviews in Hematology, 2021

2020

The diversity of genetic outcomes from CRISPR/Cas gene editing is regulated by the length of the symmetrical donor DNA template

Authors: Hewes AM, Sansbury BM, Kmiec EB

Journal: Genes (Tenth Anniversary Issue), 2020

Modeling pediatric AML FLT3 mutations using CRISPR/Cas12a- mediated gene editing

Authors: Rivera-Torres N, Banas K, Kmiec EB

Journal: Leukemia & Lymphoma, 2020

gRNA sequence heterology tolerance catalyzed by CRISPR/Cas in an in vitro homology directed repair reaction

Authors: Hewes AM, Sansbury BM, Barth S, Tarcic G, Kmiec EB

Journal: Molecular Therapy – Nucleic Acids, 2020

Potential Inequities in New Medical Technologies

Authors: Kmiec EB, Marron J

Journal: Scientific American, 2020

Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells

Authors: Banas K, Rivera-Torres N, Bialk P, Yoo BC, Kmiec EB

Journal: Molecular Cancer Research, 2020

2019

Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas

Authors: Sansbury BM, Hewes AM, Tharp OM, Masciarelli SB, Kaouser S, Kmiec EB

Journal: Nature, Scientific Reports, 2019

CRISPR-Directed Gene Editing in a Community Cancer Center

Authors: Kmiec EB

Journal: Oncology Issues, 2019

2018

Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells in Vitro and in Xenograft Mouse Model

Authors: Bialk P, Wang Y, Banas K, Kmiec EB

Journal: Molecular Therapy Oncolytics, 2018

Efficient Delivery and Nuclear Uptake Is Not Sufficient to Detect Gene Editing in CD34+ Cells Directed by a Ribonucleoprotein Complex

Authors: Modarai SR, Man D, Bialk P, Rivera-Torres N, Bloh K, Kmiec EB

Journal: Molecular Therapy – Nucleic Acids, 2018

CRISPR-directed gene editing in vitro catalyzed by Cpf1 (Cas12a) nuclease and a mammalian cell-free extract

Authors: Sansbury BM, Wagner AM, Nitzan E, Tarcic G, Kmiec EB

Journal: CRISPR Journal, 2018

2017

A Standard Methodology to Examine On-site Mutagenecity as a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and ssODN in Human Cells

Authors: Rivera-Torres N, Kmiec EB

Journal: Journal of Visualized Experiments (JoVE), 2017

Allelic analysis of on-target CRISPR/Cas9 heterogeneity by the focused disruption and reassignment of the GATA1 initiation codon in ML-DS

Authors: Bloh K, Bialk P, Gopalakrishnapillai A, Kolb EA, Kmiec EB

Journal: Molecular Therapy – Nucleic Acids, 2017

To Cleave or Not to Cleave: Therapeutic Editing with and without Programmable Nucleases

Authors: Woolf TM, Channabasavaiah GB, Boyce F, Kmiec EB

Journal: Nature Reviews Drug Discovery, 2017

Insertional mutagenesis by CRISPR/Cas9 Ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides

Authors: Rivera-Torres N, Banas K, Bialk PB, Bloh K, Kmiec EB

Journal: PLOS-ONE, 2017

2016

Personalized models reveal mechanistic and therapeutic insights into CEP290-associated Leber congenital amaurosis

Authors: McDougald DS, Kmiec EB, Mills JA

Journal: Stem Cell Investigation, 2016

Gene Editing for Cancer is Coming of Age

Authors: Kmiec EB

Journal: Oncology Times, 2016

Analysis of point mutation repair and allelic heterogeneity generated by CRISP/Cas9 and single-stranded DNA oligonucleotides

Authors: Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Dula M, Kmiec EB

Journal: Nature, Scientific Reports, 2016

2015

Genetic spellchecking: gene editing using single-stranded DNA oligonucleotides

Authors: Rivera-Torres N, Kmiec EB

Journal: Plant Biotech Journal, 2015

Regulation of gene editing activity directed by single-stranded oligonucleotides and CRISPR/Cas9 systems

Authors: Bialk P, Rivera-Torres N, Strouse B, Kmiec EB

Journal: PLOS-ONE, 2015

Is the age of genetic surgery finally upon us?

Authors: Kmiec EB

Journal: Surgical Oncology, 2015

2014

The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-Stranded Oligonucleotides

Authors: Rivera-Torres N, Strouse B, Bialk P, Niamat R and Kmiec EB

Journal: PLOS-ONE, 2014

Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds

Authors: Sims-Mourtada J, Niamat R, Samuel S, Eskridge C and Kmiec EB

Journal: International Journal of Nanomedicine, 2014

Combinatorial gene editing in mammalian cells using ssODNs and TALENs

Authors: Strouse B, Bialk P, Niamat R, Rivera-Torres N and Kmiec EB

Journal: Nature, Scientific Reports, 2014

2013

Electrospun fiber membranes enable proliferation of genetically modified cells

Authors: Borjigin M, Eskridge C, Niamat, R, Strouse, B, Bialk P and Kmiec EB

Journal: International Journal of Nanomedicine, 2013

2012

Proliferation of Genetically Modified Human Cells on Electrospun Nanofiber Scaffolds

Authors: Borjigin M, Strouse B, Niamat R, Bialk P, Eskridge C, Xie J and Kmiec EB

Journal: Molecular Therapy – Nucleic Acids, 2012

Oligonucleotide Delivery by Nucleofection Does Not Rescue the Reduced Proliferation Phenotype of Gene-Edited Cells

Authors: Livingston P, Strouse B, Perry H, Borjigin M, Bialk P and Kmiec EB

Journal: Nucleic Acid Therapeutics, 2012

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